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The composition of genes in each of the multiplexes and the primer sequences are shown in Additional file 1: Table S1.
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Findings in one affected individual, randomly selected from each of the multiplex families, were used in the calculations.
For each multiplex PCR reaction, Tm was optimized: 56°C for multiplex 1, 60°C for multiplex 2, 57°C for multiplex 3 and 60°C for multiplex 4. Each of the multiplex PCR products was subsequently genotyped in a corresponding multiplex SBE reaction and analyzed on an ABI Prism® 3100 genetic analyzer using Genescan™ Analysis software (Version 3.7).
One dream: to be the king of the multiplexes.
Neither the managers of the multiplex nor executives of National Amusements, the Massachusetts company that built the multiplex, could be reached for comment.
These graphs represent the read count spread of primer pairs within each of the four multiplex primer pools tested.
The final cross-validated results of the SBE amplifications are presented in Table 1, and show that all target SNPs were determined for each sample, demonstrating the robustness of the multiplex methodology.
Before the advent of the multiplex, it was the greatest show in town.
But war profiteer of the year must be that Chomsky of the multiplex, Adam Sandler.
Times move on, fashions change, and 3D cartoons have the run of the multiplex.
Such conservation efforts have not slowed the spread of the multiplex.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com