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The sequence data representing the CDS in each of the insert sequences were generated from at least two reads.
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The volumes and activities of each of the inserts and acquisition times are summarized in Table 2.
100µl peptide solution was loaded directly into each of the inserts and then incubated for at least 1 hour at 37°C for gelation.
Mice were assigned to either towel or foam insert groups which signified which cue was associated with reward, and this was counterbalanced with respect to group, such that equal numbers of knockout and wildtype animals were trained to each of the inserts.
One sequence attempt was made from each end of the insert fragment for each of the individual clones.
In each well of the insert, 50,000 cells were seeded.
Short sequences from each end of the insert were extracted to generate two reads.
About 20 000 cells were seeded on each side of the insert and incubated for 48 h.
Each end of the insert contained the last intron and exon of an LWS opsin gene and approximately 790 bp beyond the stop codon.
Because students produce clones with the 2.6-kb insert in both directions, there are two sets of mapping digests made and separated through the same large agarose gel, a total of 10 digests for each orientation of the insert.
The orientation of each insert within a goal arm changed in a pseudo-random order from session to session, so that each end of the insert was equally often located either at the end of the goal arm furthest from the start arm or at the choice point in the start arm.
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