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All of the EBC terms in Eq. (1) were directly measured at each of the EC towers (Zhang et al. 2007).
Signals were referenced with electronically linked mastoid electrodes (TP9/10) or a scalp vertex (Cz) electrode to yield two data sets for each of the EC and EO recordings.
Two separate pools of total RNA were isolated from each of the EC, EQ, FP, and FC regions microdissected from 100 lenses, and the resulting total RNA preparations were subjected to Illumina mRNA directional library preparation followed by high-throughput RNA-sequencing.
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The first was aimed at analyzing each frequency band individually during each of the EO and EC conditions (for each of the mastoid and Cz referenced datasets).
We performed the analysis on a region 2 kb upstream to 100 bp downstream of each of the EC-restricted genes using vertebrate_non_redundant matrices (yes set).
A colorogram demonstrating the expression pattern for each of the EC-restricted genes is shown in Figure 2. The colorogram consists of a range of patterns from transcripts highly expressed in all EC types (Pattern IV) to transcripts that are highly expressed in particular EC types (Pattern I).
This hypothesis was examined by querying the EC numbers of the peptides (from Type1_11-81aa) (Table 2) and the EC numbers of the 'reference set' (see Methods section) proteins to which they align and developing EC number similarity profiles at each level of the EC hierarchy.
The concentration range of the EC of each food or beverage type was found for soybean paste (0.9-2.7 0.9-2.7 red pepper paste (0.7-2.3 μg/kg), soy sauce (0.4-8.9 μg/L), and beveredes (not detected-85.8 μg/L).
Two solar panels were built at each station for the power supply of the EC stations and the data transmission unit for an automatic transfer of the measurements on the daily basis.
Briefly, at the end of each experiment, the EC [Ca2+]i was calibrated by applying ionomycin (5 μmol/L) in the presence of 5 mmol/L EGTA, followed by 10 mmol/L CaCl2.
In order to determine the full complement of MHC class I genes in each of the three ECs, we prepared cDNA libraries of MHC class I sequences from each animal.
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