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About 100 ng each of the converted DNA was amplified with primers designed for a consensus IAP LTR (GenBank accession no. M17551) [22] and the skeletal α-actin promoter (accession no. M12347).
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The HMMer-2 hmmsearch program was used to search each of these converted HMM files against the relevant test region (mm9 chr13:9,000,000-10,000,000 or hg18 chg18:1-1,000,000).
Total RNA was isolated from each of the samples, converted into cDNA and subjected to real-time PCR analysis cThe relative fold changes were calculated by 2 ddCT method in comparison to expression in buffer treated leaves.
We used POInT (Polyploidy Orthology Inference Tool) to estimate, for each of the potentially converted histone genes, its ortholog in its nearest neighboring genome.
Given these conditions, we considered an investor who was investing US$10,000.00 in each asset of the country, converted at the exchange rate on June 24 , 2016
For clarity, the EC50 value for each of the sample was converted into antiradical power, using the following equation antiradical power=1/EC50 × 100.
Raw scores for each of the subtests were converted into age-adjusted scaled scores (11), which were used to calculate a total score.
Each of the disaccharides was converted to a glycosyl donor by a selective hydrazine acetate-mediated deacetylation of the anomeric acetate followed by activation with diphenyl chlorophosphate in the presence of DMAP and Et3N to yield the α-glycosyl diphenyl phosphates 39, 40, 50, 60, 67, and 68 in 17 76% yields over two steps.
The colour of each pixel was converted into the corresponding brightness (the arithmetic mean of the red, green, and blue colour coordinates in the RGB colour space), and the scene luminance distribution was computed based on the empirically determined function between luminance and brightness.
The sum of the score of each question was converted into a total score, with the maximum of 4 (Cronbach's alpha, 0.63).
The intensity of each spot in each group was then normalized to the median intensity of its group, i.e. the intensity of each spot was converted into the intensity it would have had if the gel it was in had a total intensity equal to the median total intensity of the group.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com