Sentence examples for each of specific from inspiring English sources

Genomic DNA (30 ηg) was PCR amplified in a 33 µl reaction with 25 U/ml AmpliTaq Gold DNA polymerase (Applied Biosystems, USA) in the presence of 33 ηg each of specific forward and reverse primers, 1× PCR Gold buffer (Applied Biosystems) containing 1.5 mM MgCl2 and 200 µM dNTP and using a programmable thermal cycler (MJ Research, Watertown, MA, USA).

Briefly, 1 μg of total RNA from each sample was added to the reaction mixture containing 1× AccessQuick master mix (Tfl DNA polymerase, AMV/ Tfl reaction buffer, 25 mM MgSO4 and 10 mM dNTP mixture), 10 μM each of specific sense and anti-sense primers, 5U AMV reverse transcriptase, and nuclease-free water to a final volume of 50 μl.

Reactions were performed in a 25 μl volume containing cDNA generated from 10 ng of original RNA template, 300 nM each of specific forward (F) and reverse (R) primers (Table 1), and 12.5 μl of iQ™ SYBR Green Supermix (Bio-Rad).

Each reaction (6 μL final volume) comprised 50 ng template DNA, 20 nM specific forward primer (tailed), 300 nM each of specific reverse primer and fluorescently labelled tail primer and 3 μL 2× MyTaq HS mix (a Taq polymerase mastermix from Bioline Reagents Ltd ,UK).

Real-time reactions were performed in duplicate containing cDNA generated from 10 ng of original RNA template, 300 nM each of specific forward and reverse primers, and 5 μl of iQ™ SYBR Green Supermix (Bio-Rad) in a 10 μl final volume.

In brief, the samples were amplified in a 6 μL reaction mixture, containing 5 ng genomic DNA, 0.3 pmol each of specific forward and reverse primers, 200 μL of each dNTP, 3.25 mM MgCl2 and 0.2 units of HotStarTaq polymerase (Qiagen, Valencia, CA).

The results indicated three-stage deoxidation process of GO, each comprising of specific surface chemistry, structural changes and electrochemical behavior.

We can use the estimated function to predict the response in each value of specific controllable factors.

Each component consists of specific elements that may have some attributes and be linked by various relationships.

Each is characteristic of specific types of hauntings.

Many of her combinations target specific health concerns and she explains clearly how each individual ingredient is of specific benefit.