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DNA was isolated from between 5 and 10 clones in each occasion, using the QIAspin Miniprep Kit according to manufacturer's protocol, and used to PCR the inserted sequence with M13 primers (forward 5'-GTTTTCCCAGTCACGAC-3', reverse 5'-CAGGAAACAGCTATGAC-3').
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Written consent from each dog owner was collected by the veterinarian on each sample occasion using a specific consent form approved by the Ethical committee.
Two tests will be conducted on each testing occasion using a standardised protocol [ 36], with the greatest distance recorded.
Tolerability was assessed daily by the clinician during each study occasion (using an adverse-event report form) and at the follow-up visit 1 week after last study occasion (using an adverse-event report form and laboratory assessment).
Test-retest reliability was determined by assessing each child on a second occasion using predominantly the same rater.
He returned to Marvel, on one occasion using the pen name Reg Everbest, and even to scripting Howard again.
Long before Orbán was battling Soros, he was fighting the Russians, on occasion using Soros money to do so.
We controlled unrest through simple methods - such as, on occasion, using the RAF to bomb rebellious tribesmen.
Many people captured the occasion using their mobile devices and cameras.
In addition to weight, body fat percentage assay was also performed in each of these occasions using a Tanita 500 bio-impedance system.
He has often collaborated with the Northwest Sinfonia orchestra from Seattle, though he has on occasion used other orchestras.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com