Bound like a thorn to the nub of each of their severed spurs is a curved, two-inch-long steel gaff that can puncture heart, brain or lungs with the thrust of a heel.
However, if Cub and the altered Nub are each linked to polypeptides that interact in vivo, the cleavage of the fusion containing Cub is restored, yielding a generally applicable assay for kinetic and equilibrium aspects of in vivo protein interactions.
To further analyze the differentially expressed transcripts found in each sample type in nub 1 versus wt, a gene set enrichment analysis was conducted identifying overrepresented Gene Ontology (GO) categories.
Recent analysis of the level of expression of each of the isoforms of nub and pdm-2 confirmed that RNA coding for the short and long isoforms are present both in the embryo and larvae [ 22].
Prestin- and cdh23- expressing yeast were cultured in SD-Leu media at 30°C over night until they reached an OD546 of 0.6. 2 μg each of pAlg5-NubI and pAlg5-NubG plasmids were transformed into prestin- and cdh23-expressing yeast according to the manufacturer's instructions (DUALmembrane kit. Biotech, Switzerland).
There would still be an unnecessary learning curve, but over time, the driver would learn where each button was located and the little nub would confirm they're on the right function.
The availability of genomic sequences from other Diptera, including 24 mosquito species, the Mediterranean fruit fly (Ceratitis capitata or medfly) and the housefly (Musca domestica), has allowed us to compare the Nub and Pdm-2 proteins in each of these species and determined their sequence relationship (Fig. 1b).
The danger they pose is, in part, because they are impediments, but also because of their deteriorating nature: after years of pounding surf and ripping current, each stick has been worn down to a barnacle-covered nub, rendered invisible by the dark blue-green water during high tides.
Each of the Ismael statues has been built with a small nub between his lips, so followers can wedge cigarettes and weed in their mouths as offerings.
