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A standard curve generated for each Msg construct was used to calculate the units of reactivity.
Similar results were found for each Msg fragment in each patient group.
It should be noted, however, that the mark-recapture approach assumes that each MSG gene was equally likely to be observed.
The protein expressed from the sequences of the pET vector is an integral part of each Msg recombinant tested, and therefore this method of analysis allows measurement of Msg-specific immune reactivity corrected internally for any reactivity to the vector-derived sequences.
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The study was carried out on 60 newborn Wistar rats, divided into 3 groups, 20 animals in each (females – n = 10, males – n = 10): intact rats, monosodium glutamate (MSG-) and MSG + probiotic group.
Irrespective of the method of analysis, HIV-positive patients had significantly higher levels of antibody than healthy blood donors to each of the Msg fragments (p < 0.001), even though the range of ELISA values overlapped between the two groups.
Irrespective of the ELISA method chosen, the reactivity of PCP-negative serum samples was higher than that of blood donor serum samples for each of the Msg fragments (p < 0.01), indicating that PCP status alone could not account for the increased antibody reactivity found in the HIV cohort.
The reactivity of each serum specimen to Msg was corrected by subtraction of the reactivity of that serum to PBS (mean OD Msg – mean OD PBS), and the results were quantified using the method of Bishop and Kovacs [34].
To evaluate the possibility that this type of error contributed to the data, two plasmids, each carrying a distinct MSG insert (the two inserts were 80% identical), were mixed together and subjected to PCR amplification.
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