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Each liver sample was immediately frozen at 20 °C until analyzed.
The average time for measuring each liver volume was 17.2 ± 6.5 min.
Each liver volume was measured on different occasions using 3-, 4-, 5-, and 6-cm grids.
On partial resection for each liver metastasis, S4 lesion resulted in positive resection margin.
Each liver voxel is then assigned to one arterial branch by a model-based approach considering the shortest distance.
After the rats were euthanized, the left lateral lobes of each liver were embedded in paraffin and thin sectioned coronally.
Microtome sections (5 μm thick) were prepared from each liver and kidney sample and stained with heamtoxylin-eosin (H&E) dye.
About 0.1 0.3 g of each liver tissue and 0.5 mg of liver DNA from various treated mice were weighed, digested, and analyzed for titanium content.
Wayel Jassem, transplant surgeon who performed both operations, said: "I was impressed to see how quickly each liver started to function following the transplant.
At least five fields per section were analyzed from each liver sample.
The expression pattern within each liver acinus forms a gradient of expression that fits a known oxygen gradient[41].
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