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Each library was inspired by structures embedded in the framework of specific alkaloid natural products.
The quality of each library was determined using a BioRad Experion (BioRad, Hercules, CA).
Each library was amplified with the primers used in the 454 sequencing emulsion PCR process.
Each library was sequenced independently.
Each library was sequenced in a single lane.
Each library was sequenced using Solexa 1× genome sequencer (Illumina, San Diego, CA).
3) Before emPCR, the DNA quantity in each library was determined using qPCR [29].
The coverage of each library was calculated using the abundance-based coverage estimator (ACE).
Each library was indexed.
Each library was sequenced in one lane.
Each library was sequenced in one region of the PicoTiterPlate.
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