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Each lever press delivers one food pellet, and pressing harder would not change the amount of food dropped into the cup, so why waste energy?
Each lever press was followed by a 0.11-s refractory period in which lever pressing was not effective.
The time of each lever press was recorded in 1-s bins.
Each lever press in the presence of an audible tone (3.5 kHz, 70 dB) produced an intravenous infusion of cocaine (0.355 mg/kg infusion), terminated the tone, and started an intertone interval (3 6 min).
Initially, each lever press was reinforced.
The program sequentially recorded the IRT of each lever press to within a hundredth of a second.
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Here each lever-press delivered only saline (0.13 ml/injection) and sessions continued for each animal until its response rate dropped to fewer than 15 responses per 4-h session; this was the baseline level of responding seen in control animals introduced to the chambers with no reinforcement.
Each lever-press generates a click of the sensor; a click within a 2-second period of the previous click was considered as one hand disinfection event [ 5, 6].
After introducing the animals (N = 20) to the behavioral apparatus and 1 day of continuous reinforcement, where each lever-press was reinforced, animals were trained intensively with three daily sessions for 3 days to perform fast lever presses.
The experiment began with one-hour sessions where each active lever press was reinforced (Fixed Ratio-1, FR-1).
Each active lever press resulted in an infusion of 0.1 ml saline (0.9% NaCl), or methylphenidate (0.25 ml/0.1 ml infusion) [ 34] for cohort 1 or 2 groups, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com