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Each isolated cell type (PαS, OP, Tail Tip Fibroblast (TTF), and Mouse Embryonic Fibroblast (MEF)) was retrovirally transduced with 4 or 3 factors [15] along with CAG-DsRed [16] as a control for the induction efficiency, which was similar for both cell populations (Figure S1).
Routinely, six CFU-F assays were performed for each isolated cell population.
Routinely, population doubling time assays were performed in triplet for each isolated cell popu-lation.
In order to control in vitro multilineage capacity differentiation experiments were conducted with cells from horse 1. Routinely, differentiation assays were performed in triplets for each isolated cell population.
In each isolated cell, the magnetite particles were next to the cell membranes, the scientists report online today in the Proceedings of the National Academy of Sciences.
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Each peak of maximum impedance corresponds to a local minimum in isolated cell number.
This scenario is called an isolated cell.
Human PV isolated cell electrophysiology has not yet been studied.
These experiments showed that these adherent cells were >93% of F4/80 positive in WT and >95% of KO cells in each 10 isolated cells.
In order to find shared aberrations between a specific biopsy and the respective isolated cells, we compared the paired genomic profiles ("biopsy" and "isolated cells") for each sample.
Freshly isolated cells from each sample were filtered through a 50 mm sterile nylon mesh and examined by means of flow cytometry.
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