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First, a list of homologs for each sequence, consisting of those whose relative BLASTP scores are less than 1e−5 over a total of at least 50% of the length of each, is generated for each protein.
The production crosses were such that the four genotypes XO, XY Sry-, XYd-1 and XYd-3 all had a maternally derived X chromosome; each is generated in a separate cross using a common pool of outbred XX mothers.
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Four biological replicates each were generated for K27me3 M+P−, K27me3 M+P+, and "wild-type" hybrid samples.
These conflicting decisions seem paradoxical because each was generated using approximately the same literature database.
Using CASAVA package, provided by Illumina, PE sequence reads of length 72 bp each were generated.
Two libraries each were generated from DNA and RNA isolated from plasma samples (Table).
Analysis began with the reading and re-reading of each transcript and a summary of each was generated.
The four DNA pools comprising 150 individuals each were generated using equimolar amounts of DNA from each individual.
Of the unique peptides, five were chymotryptic fragments, while two each were generated with thermolysin and trypsin.
Large randomization blocks with a size of 50 participants each were generated on Microsoft Excel (with the Random function).
Given the positions and reputations of these two players, they might not each be generating the exact same kind of fear.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com