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Our phylogenetic tree only showed a difference in that while sub-families 3 and 4 are also here each inferred as independent monophyletic clusters, this inference is without high supporting values.

We randomly selected one offspring representing each inferred mother for further analyses.

Petrographic characteristics of the silicic vitrophyres indicate that crystal-poor magmas underwent distinct cooling paths for each inferred conduit area.

To estimate the individual confidence of each inferred parameter, we employed a bootstrap method [21].

All the positions were mapped onto the pig pancreatic amylase protein sequence (Fig. 2). Figure S1 shows the mapping of intron positions onto a protein alignment, showing the conservation of the amylase sequence at each inferred position.

A ρ value is observed for each inferred breakpoint in an alignment, so long as that breakpoint is associated with a domon boundary (and hence with a domon) annotated in at least one of the aligned sequences.

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The results also include files containing the estimated parameters for the model used, the mean posterior rates for each family and the probabilities for each of the inferred ancestral family sizes.

We estimated overall Ne for simulations (assumed equal to ancestral Ne) by summing female Ne (Nef) estimates from θ values, calculated for each SAMOVA-inferred population using the equation θ = 2 Neμ (for mtDNA, μ = cyt b rate estimated in BEAST).

For each TF, the inferred activity profile over roughly a thousand conditions represents a highly specific regulatory signature.

Each of the inferred subpopulations was then tested for agreement with Hardy-Weinberg predictions and although none of the four subpopulations show agreement for all loci, some loci are in agreement with the values predicted for a randomly mating population (Table S5).

The phylogenetic relationship between A. allosaccharophila (strain LMG140549T) and members of AVG was investigated in the same way for the concatenation of housekeeping genes and the chitinase gene and for each of the inferred recombinant fragments of aexU using A. hydrophila as an outgroup to root the trees.

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