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Two independent PCRs were performed for each individual studied.
Thus for each individual studied, it was usual to measure at least seven time points.
First, we used PLINK to extract the genotype information of each SNP probe for each individual studied.
The methylation cluster analysis for each individual studied gene is illustrated in Additional file 2. Hierarchical cluster analysis profiling of the promoter alterations and the significance of the alterations are shown in Figure 2(a) for the 12 studied genes.
Global climate models developed for GISs [ 42] link climatic information with sampled individuals, providing both quantitative environmental data for each individual studied and a means for controlling spatial bias over genetic diversity.
Based on allele frequencies, this approach identifies genetically distinct populations by estimating, for each individual studied, in this case each leishmanial strain, the fraction of the genotype belonging solely to it.
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Furthermore, the methodological quality of each individual study was evaluated by using the MMAT [18].
Estimates were also obtained for each individual study region and year.
The frequency-weighted mean represents the mean from each individual study weighted by the number of patients in that study.
It will therefore be necessary to clarify what scientific knowledge can be acquired in accordance with each individual study.
Calculation of effect size for each individual study, pooled estimates, and estimates of heterogeneity across studies was carried out using the software Comprehensive Meta Analysis (version 2.2.064) [32].
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