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The experiment was performed in triplicate, where each individual spectrum is plotted in grey and the mean of the three measurements is plotted in red.
In other words, the OH bands in the artificial spectrum were considered to be simply a sum of hydrogen-bond absorptions contributed by each individual spectrum.
One hundred points and 2-s acquisition time were used for each individual spectrum.
In this context, unmixing is defined as the problem of determining the individual spectra, given measurements of multiple spectra that are spatially resolved across samples, as well as the determination of the corresponding abundance maps indicating the local weighting of each individual spectrum.
Under the assumption that the spectrum of an individual fluorophore is linearly summated on each image pixel, SU extracts the weight (or contribution) of each individual spectrum, with that weight proportional to the fluorophore concentration [14].
Each individual spectrum is described as a combination of these pure components by the spectrum's scores on each component.
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To determine the contribution of each of the tryptophan residues to the overall spectrum, we deconvoluted the spectrum obtained by subtracting each individual tryptophan spectrum from that of the wild type spectrum (Fig 1C).
In this mean spectrum and in each individual sample spectrum, signals from three distinct domains of T2 were readily identified, as previously found [5]: 50 µs<T2<150 µs, defined as pool A, due primarily to collagen methylene protons; 150 µs<T2<1 ms, pool B, due primarily to collagen-bound water protons; and 1 ms<T2<1 s, pool C, due to water protons in pores in lipid protons.
However, even more detrimental to its general use is that each FID is composed of several data chunks, usually around 30, each recorded in an individual spectrum.
To set a criterion for the robustness of peaks for acceptance in subsequent analyses, the values in each individual amplitude spectrum were expressed as a proportion of the maximum value between 0 4000 Hz in that amplitude spectrum, and spectra were rms-averaged across all 22 participants for each stimulus type.
All lines were resistant to adriamycin, but each line appeared to have an individual spectrum of sensitivity to the more effective drugs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com