The quest for the testimonies left by the earthquake observers, i.e. those testimonies which withstood the passing of centuries, is just the first step of a systematic and painstaking reconstruction of what actually each individual place experienced the moment when the earth quaked, and its aftermath.
+ Also, an unknown individual placed "White Elevators" signs around the convention with a Jim Crof-sense of irony.
Individuals of both species were shown a demonstration in which one individual placed a ball into one of the holes three times, whereas three other individuals each placed a ball into a different hole once each.
For the preliminary trial, each individual was placed into its own plastic colander (20 cm diameter).
Larvae were killed with an overdose MS-222 (tricaine mesylate, Sigma-Aldrich, St . Louis USA), and the region between head and pectoral fin from each individual was placed in Eppendorf tubes, snap-frozen, and stored at −80 °C.
Following application of the stimulus, each individual was placed in a small tank for observation.
Approximately 1 2 ml of blood were taken from the caudal vein of each individual and placed in storage buffer (100 mM Tris, 100 mM EDTA, 2% SDS).
Each individual was placed in a 0.2 ml microtube containing 10 µl of a lysis buffer (Tris-HCl 10 mM pH 8.2, KCl 50 mM, MgCl2 2.5 mM, Tween-20 0.45%, gelatin 0.01%, proteinase K 60 µg/ml).
The generation of these networks is described fully in a previous manuscript [5]; briefly, following the method of Read & Keeling [6], we generate a population in which each individual is placed at random on a square patch at a constant average density.
Each individual was placed in a 100-ml jar filled with Daphnia medium (ADaM).
