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As such, the use of transgenic lines precludes the need for other costly and labor-intensive procedures to achieve functional transgene expression in each individual experimental animal.
In order to evaluate proliferation rates, 10% (v/v) of each individual experimental setup from passage 3 were seeded into 96 well plates in triplicate.
CNQX reduced the mean slope of the PSP vs. membrane potential relationship for all recordings by 89% (Figure 1D; Figure S2 displays this data for each individual experimental group).
Extracted miRNAs were 3' end labeled with pCp-Cyanine dyes (pCp-Cy3 for common reference, which was a mixture of all hybridized samples and pCp-Cy5 for each individual experimental sample).
It is recommended that an optimal set of reference genes be identified for each individual experimental setting or tissue.
To produce sufficient DNA for sequencing, while minimising PCR amplification, PCR repeats were performed for each individual experimental sample.
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Each of the individual experimental runs at a given particular ratio and temperature was monitored and samples were collected at reaction times intervals of 1, 2, 3, 4 and 5 h for analyses.
Specifically, each assertion describes an individual experimental observation from an experiment performed in a human, mouse, and rat species context, either in vitro or in vivo.
Specifically, each assertion describes an individual experimental observation from an experiment performed in a human, mouse or rat species context, either in vitro or in vivo.
Carrying out the experiment according to the schedule shown in Fig. 5 required approximately 2 h per respondent, and only three or four people could be tested each day because of the individual experimental design.
Individual experimental currents from each segment were summed to determine total current, and compared with the theoretically derived value.
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