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Each group was aligned using ProMals (Pei and Grishin, 2007 ).
Then, each group was aligned separately using Muscle version 3.8.31 (Edgar 2004), and the aligned sequences of all the groups were concatenated and conserved blocks were obtained for phylogenetic analysis using Gblocks version 0.91 b (Castresana 2000) with default parameters.
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Structures in each group were aligned by the multiple structure alignment function of RSmatch with default scoring matrices.
The proteins in each group were aligned using the Darwin multiple sequence alignment package (Gonnet, Hallett, et al. 2000; Gonnet, Korostensky, and Benner 2000).
The AUG 3' flanking sequences in each group were aligned and analyzed by the Weblogo program.
Sequences in each group were aligned (using ClustalW [30] and Dialign [31]) for careful inspection of their features.
The sequences of each group were aligned to obtain sequence logo of the translation initiation site using WebLogo 3, version 2.8.2 (http://weblogo.berkeley.edu/).edu/
The proteins within each group were aligned against that group's representative PDB sequence.
Following the clustering, sequences within each group were aligned using MUSCLE.
The sequences of each group were aligned using MUSCLE (Edgar 2004).
Amino acid sequences in each group were aligned using MAFFT 7.045b under default settings [ 47].
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