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The figures showed that the protective activity of each fractions varies with the enzyme assayed for.
The chloroform fraction (20 g) was subjected to silica gel column chromatography (60 120 mesh) and the column (1.2 m length and 4 cm in diameter made of glass was used) was eluted with hexane ethyl acetate (99 01 0:100) affording 48 fractions (each fractions of 200 mL volume).
80 µg of each fractions were analysed by SDS- PAGE followed by Western blotting using SREBP-1 monoclonal antibody.
For peptides identification, each fractions presenting a nerve sprouting activity were further loaded onto a C18 column (250×2.1 mm, 218TP52 Vydac™) with a gradient consisting in 2 25% AcN in acidified water for 10 min and 25 35% AcN for 40 min at a flow rate of 0.2 ml/min.
Each fractions was precipitated overnight at 4°C in 2 volumes of absolute ethanol and analysed by western blot.
Cell growth occurs between each fractions of RT, so this effect must be counteracted in the cell killing model.
Similar(54)
α-Tubulin in each fraction was determined by western blot.
Each fraction, containing multiple frames, represented a dose of ~ 2 electrons/Å2.
The absorbance at 280 nm was measured for each fraction, and protein-containing fractions were pooled.
Each fraction will then be tested in mice to see if they boost brain function.
Each fraction was monitored by Western blot using antibody against PML (Abcam).
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