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The velocity of each foci was measured from displacements of >5 successive frames by a least-squares curve-fitting procedure.
In case of a single masse lesion with a fragmented response, i) the overall dimension of the foci is given when foci are adjacent, ii) each foci is measured when foci are distant and the overall sum is given.
The number of metastatic foci was counted in lung after staining with H&E and immunostaining with CK7, and the size of each foci was evaluated by measuring its diameter (in μm) using the Olympus DP-Soft morphometry program in an Olympus DX51 microscope.
Specifically, we noted the direction of relative activation levels (positive or negative) compared with rest/fixation for each foci contributing to the significant clusters in the primary ALE analysis (if this information was reported in the original paper see Supplementary Material for a summary regarding the proportion of studies that reported this information).
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I could feel my heartbeat slow with each focused inhalation and exhalation.
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These reports each focus on a different aspect of the same story.
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