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An extraction blank was included in each extraction procedures to control the cross-contamination between extracts.
The resulting triglycerides and fatty acids from each extraction are modeled in the pre-treatment process.
After each extraction, the residue was submitted to leaching essays, to assess environmental risks.
Main Outcome Measure: Amount of toxin left in vials after each extraction method.
The solvent from each extraction was partially evaporated and the fatty acids methylated by methanolic HCl.
Each extraction experiment was performed in triplicate.
Each extraction operation lasted for 10 min.
Each extraction was done in duplicate.
Each extraction method had their own pros and cons.
Each extraction was carried out for at least 1 h.
The supernatant from each extraction was dialyzed as described above.
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