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Afterwards each DNA was quantified using fluorimetry (Quant-It DNA Assay Kit, Invitrogen, Carlsbad, CA, USA) and checked for quality using 1% agarose gel electrophoresis.
The concentration of each DNA was quantified and qualified spectrophotometrically using a Nanodrop HPV genotyping was determined in all study samples using the INNO-LiPA HPV Genotyping Extra assay (Innogenetics, Belgium) and following the manufacturer's instructions.
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Concentration of each library DNA was quantified using KAPA Library Quantification Kits (Roche) according to the manufacturer's instructions.
The amount of immunoprecipitated DNA was quantified relative to the amount of the input DNA for each sample.
Genomic DNA was quantified by Quantifiler Human DNA Quantification kit (Applied Biosystems).
Pellets were resuspended and the resulting DNA was quantified on a NanoDrop spectrophotometer.
After eluting the sample in 100 μl of distilled water, double-stranded DNA was quantified.
DNA was quantified using the Qubit® dsDNA HS Assay Kit (Life Technologies; Q32854).
Genomic DNA was quantified using a Qubit 2.0 Fluorometer, and quality-assessed on an Agilent 2200 Tape-Station D1000 ScreenTape.
BALF supernatant double-stranded DNA was quantified using Quant-iT PicoGreen® (Invitrogen, Canada) following the manufacturer's protocol.
DNA was quantified with a Light Cycler 480 (Roche) and Light Cycler 480 release 1.5.1.62 SP software (Roche) using FastStart DNA Essential DNA Green Master (Roche).
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