To assess the clonal origin of each prostatosphere, dissociated single cells were stained with the fluorescent dye CellTracker green or CellTracker red (Molecular Probes/Invitrogen) at a concentration of 5 μM in separate reactions, following manufacturer instructions.
Findings suggested that assessment had an impact on how students interacted online and in their use of rhetorical moves; and that knowing and learning are related but distinct constructs, correlated within each writing context, dissociated across contexts, and performing differentially as a function of students' perceptions of academic demands.
After confluence had been reached, the cells in each dish were dissociated by trypsin treatment and plated in new dishes.
Spheres were dissociated each week with a renewal of two third of the culture medium, as previously described.
Therefore, they proposed a new rating system that dissociated each of the three following dimensions of hip function: pain, ability to walk, and mobility, in seven ordered categories (Table 1).
In this case, we obtained 11 embryos at E4.5 by crossing Blimp1+/− heterozygous mice and repeated the procedure for ESC derivation described above, except that the ICM from each embryo was dissociated into single cells and then dispersed onto a feeder layer in a 48-well culture dish.
However, some evidence suggests that the conscious experience of mental imagery and short-term visuo-spatial retention can be dissociated from each other, implying at least a partial dissociation between the cognitive processes that underlie each function (Pearson, 2007; Zeman et al., 2010).
Given that single VM were required for each culture, and the low yield of neurons generated, each dissected VM was dissociated as describe above and plated into a 96-well plate (50,000 cells/well).
In either case, the addition of agonists resulted in dimeric forms of each LBD dissociating into monomeric forms.
