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Each differentiation medium was replaced every 2 3 days for 21 days.
Error bars indicate the standard deviation for 3 biological replicates at each differentiation time point.
Ninety-one days after transfection (2 weeks after the stimulus for each differentiation), osteocalcin expression was induced, but neither FABP nor aggrecan were expressed (Figure 5b).
Constructs composed of AFSC-SPCL scaffolds from each differentiation stage were implanted into critical sized femoral defects.
After each differentiation, we eliminate D q Z ( t ) by (20) and we take into account (10 - 11 10 - 11
We then wanted to test if the change of integrin β1 is accompanied with the change of cell rigidity, and we did so using AFM to measure cell Young's modulus of each differentiation stage.
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While integration refers to the ability of the elements of a system to interact with each other, differentiation may be defined as the system's ability to react in different ways to different perturbations.
As we assume that the cross-inhibition genes stand at the branching point of each cell differentiation, a pair of opposing TFs, MafA <- ->δ factor, is proposed to govern the determination of β vs. δ cells respectively.
MSCs were plated and grown with each specific differentiation media (from Miltenyi Biotec).
Therefore, upon differentiation, each HSC produces one partially differentiated progenitor cell and one replacement HSC.
Additionally, they often have a heavy client load, which makes it challenging to spend enough time researching and speaking with each client to truly understand the value and differentiation each company provides.
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