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In samples containing virus, the PCR yielded DNA products, the unique sizes of which were diagnostic for each dengue serotype.
There are two glycosylated asparagines on each dengue E subunit: Asn-153 on domain I and Asn-67 on domain II.
However, only few linear immunogenic determinants on the surface of the virion of each dengue serotype have been identified and a detailed map of the dengue linear B-cell epitopes still need to be determined [14].
Plates were incubated at 37°C in an atmosphere of 5% CO2 for an additional 4 6 days, depending on the growth kinetics of each dengue virus serotype.
Information for each dengue case includes patient information (e.g., sex, age, race, and neighborhood), diagnosis date, first symptoms date, hospitalization date (if any), final condition, and reporting institution.
21– 23 Briefly, serial twofold dilutions of heat-inactivated serum were mixed with a constant challenge dose of each dengue virus.
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One non-dengue fever control was assigned to each acute dengue case; each was recruited from the surveillance study using diagnostic laboratory results.
Five patients experienced six serious infections (one each of dengue fever, erysipelas, gastroenteritis, herpes zoster, bacterial meningitis, and pyelonephritis) [ 19].
At scheduled visits within 30 days after each dose, dengue viremia was detected in five TDEN recipients.
Each year dengue causes 24 000 deaths, 250 000-500 000 cases of haemorrhagic fever, and up to 50 million cases of dengue fever. 2 3 The global burden of dengue for the year 2001 was estimated to be 528 000 disability adjusted life years (DALYs).
Note how each significant dengue outbreak was preceded by multiple weeks (or months) when extreme daily temperatures were largely confined within the optimal 18 32°C range.
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