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Twelve tests for each construct were performed.
Each construct was transiently transfected into HEK293 cells.
Each construct was experimentally tested using a cantilever bending approach.
Moreover, the addition of external MSCs to the bioscaffold standardise the cell quantity in each construct.
Three plants of each construct and WT were used for the measurements.
Each construct was injected into Nacre embryos alongside Tol2 mRNA at 25 pg/nl.
For each construct, inserts and their surroundings were checked by double strand sequencing.
The FLuc RLuc ratio for each construct with Flag MS2 expression was set to 1. Data are mean ± s.d.
Each construct has been optimized twice, for shape and angle.
We also computed the composite reliability (CR) for each construct.
The mode of failure of each construct was also recorded.
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