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Larvae were treated with prazosin over range of concentrations (n = 12 for each concentration) on the morning of day 5 of development.
Cell viability assay was performed using MTT assay to determine the suitability of each concentration of the β-sitosterol on the TGF- β1 treated LX-2 cells.
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Under double-blind conditions and according to a Latin square design, patients received sucrose in solution (0, 5, 10, 20, and 40%) and rated each concentration for pleasantness on a nine-point scale.
Five neonates (younger than 24 h old) were used for each concentration based on 4 replications.
Hydrothermal pretreatments were performed with solid loadings of 0.5%, 1% and 2% w/w, and dilute sulfuric acid pretreatments with 0.5% and 2% w/w solids concentrations, with each concentration based on the mass of raw biomass before pretreatment.
Blank urine samples were fortified at 0.5, 1.0, and 2.0 times the VL level for all the target analytes and seven replicates of each concentration were analysed on one day.
The impurities were co-fed with syngas, and the influence of each contaminant concentration on Fe catalyst behavior was examined for 72 144 h.
The individual influence of each measured concentration on the whole model was examined using the size-adjusted Cook's distance [ 49].
Cell viability was determined by trypan blue exclusion of cells from an additional nonstimulated replicate of each contaminant concentration on day 5 of the exposure.
