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Each bladder — the gas sack that regulates the buoyancy of the fish — reportedly fetches $10,000 dollars in the Chinese market, where it is regarded as a delicacy.
Each bladder was washed 3 times with sterile PBS.
Each bladder cancer specimen was confirmed by pathological analysis of a part of the tissue sample in fresh frozen sections from TUR specimens, and was then frozen in liquid nitrogen and stored at −80°C until use.
The number of colony-forming units of C. albicans cells that adhered to each bladder was determined by plating serially diluted samples (in PBS) onto YPD agar that contained 50 µg/ml ampicillin and incubating the plates at 30°C overnight.
Three slices from each bladder were embedded.
Mice received intravesical instillation of each bladder cancer cell line.
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For Ep-ICD, the number of tissues analyzed were 38 breast cancers and 25 corresponding normal tissues, 49 prostate cancers and 9 corresponding normal tissues and 21 benign prostate hyperplasias, 57 HNSCCs and 20 corresponding normal tissues, 46 ESCCs and 20 corresponding normal tissues, 59 each of lung and colon cancers, 10 each of bladder, ovarian and pancreatic cancers, and 9 liver cancers.
He estimated that the materials and costs for workers would be $7,000 in each experimental bladder case but noted that the figure did not include costs for hospital stays and doctors' fees.
We have reported that acellular dermal biomatrix can be used successfully for directing the regeneration of each key bladder wall element in healthy domestic pigs.
Hence, in each patient, bladder pressure volume (PV) curves could be obtained, illustrating the relative bladder compliance at that moment.
The eggs laid in each swim bladder were collected and suspended in a cap filled with 40 ml tap water.
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