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n = 6 with three independent experiments conducted for each assay.
We ran each assay in duplicate to test reproducibility.
Complementary deoxyribonulcleic acid was produced slightly differently for each assay.
n = 3 with three independent experiments conducted for each assay.
Detection limit was determined for each assay.
As a result, we proceeded to perform each assay within 30 cycles of PCR.
To confirm the accuracy of the standard curves, each assay was run in triplicate.
Before each assay, animals were acclimated to the experimental conditions for 3 days (once per day).
Each assay was performed triplicate.
Fresh solutions were prepared before each assay.
Three replicates of each assay were performed.
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