Sentence examples for each and transferred from inspiring English sources

Briefly, third instar larvae were dissected in PBS and fixed in 0.1 M PIPES, pH6.9, 1 mM EDTA, 1% Triton X-100, 2 mM MgSO4, 1% formaldehyde for 30 min. Samples were then washed three times in PBS+0.1% Triton X-100, twice in PBS+0.5% Triton (10 min each) and transferred to permeabiliation solution (sodium citrate 0.1 M in PBT) for 30 min at 65°C.

Pellets were resuspended in 50 100 μl PBS each and transferred to fresh tubes.

Subsequently, they were divided into two groups of five to six fish each and transferred to a new aquarium.

One day after, these flies were distributed in forty vials (ten males and ten females each) and transferred to the four experimental temperatures (ten vials for each temperature).

Labeled standards were mixed with unlabeled samples (300 μL each) and transferred to 5 mm NMR tubes (Wilmad) for spectroscopic analysis.

Coverslips were washed three times in sodium cacodylate buffer (0.1 M, pH 7.4) for 20 min each and transferred to osmium tetroxide (1% v v-1) in sodium cacodylate buffer (0.1 M, pH 7.4) for 1 h.

For conditioned media experiments, media was collected from non-depleted cells each day and transferred to depleted cells after each media change.

A single animal from the offspring of each of the 15 singles was randomly picked for each generation and transferred to a new plate (Supplementary Figure S3A).

One 0.6 mm tissue core had been punched out from the index tumor of each case, and transferred into a TMA format as described34.

We assume that the relatively strong synchronization of the individually isolated bees in this experiment compared with the other experiments was because their cages were placed close to each other and transferred cues such as vibrations or volatile odours.

Then, 100 μL was removed from each well and transferred to a 96-well plate.