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Each analytical method returns a discrete numerical (or other) results as well as a color.
Systematic benchmarking based on gold standard data would inform the biomedical research community of the strengths and weaknesses associated with each analytical tool available in computational biology9.
Each analytical test was performed by at least two independent laboratories using the protocols adopted by the individual organizations.
Internal standards recovery was assessed for each analytical sequence to monitor the method performance.
Each analytical run was prepared and assayed within a 28-h period.
A comparison of each analytical step in the two procedures is presented in Figure 1.
A sample of reference materials and blank was included in each analytical batch.
Background intensity computed from a set of negative controls was subtracted from each analytical data point.
after subtracting background intensity, which was computed from negative controls, from each analytical data point.
Calibration for all elements was performed at the beginning of each analytical session, followed by the analysis of known compounds as standard checks.
A standard and a blank were analysed between every 15 samples during each analytical run to permit regular autozero and calibration reslope.
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