Sentence examples for each amplification of from inspiring English sources

In each amplification of single cells from a sample, we also included one to four negative controls.

Two μg of pooled total RNA from two samples (4 hypothalami total) were then used for each amplification of mRNA using a modification of the Eberwine procedure [ 40] through the Amino Allyl MessageAmp™ II aRNA Amplification Kit (Ambion, Austin, TX).

Melting-curve analyses were applied in each amplification to test the specificity of amplification.

After the indicated cycles of each amplification, 10 μl of each PCR reaction was separated on a 2.0% agarose gel with ethidium bromide.

In each reaction, amplification of the beta-globin gene was used as an internal standard.

For each amplification reaction, analysis of the product dissociation curve was performed to exclude the presence of non-specific amplification.

The actual production, however, depends of the efficacy of each amplification cycle.

Melting curves were analyzed at the end of the reactions to verify the specificity of each amplification.

Fluorescence was measured at the end of each amplification cycle.

Melting curves for each amplicon were then analyzed to verify the specificity of each amplification reaction.

Dissociation curves for each amplicon were then analyzed to verify the specificity of each amplification reaction.