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Each alteration was confirmed at least twice by PCR.
Altered bands were checked at least thrice for reproducibility and each alteration was scored by two independent observers.
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To analyze the differences in placental histological findings among groups, the percentages obtained for each alteration were compared.
Each alteration is annotated through some features - that vary according to the type of molecular alteration - such as the contig number, the direction of gene expression variation (up/down), the experimental method used, the cancer type or the cell line.
To statistically assess the temporal pattern of the genetic lesions, we use the binomial test to assess significance by assuming that the number of in-degree and out-degree of each alteration are randomly distributed.
Because the size of each individual chromosome varies, the frequency for each chromosomal alteration was normalized for relative size.
It was found that the influence of two backbone modifications in the substrate structure was not described additively, i.e. the effect of each structural alteration was dependent upon the position of the second modification.
pCR, pathological complete response Each molecular alteration was then evaluated for its impact on overall survival.
Each sequence alteration was confirmed by sequencing both DNA strands of two independent PCR products.
Each identified sequence alteration was confirmed by the analysis of a second blood sample.
Each newly found sequence alteration was confirmed by sequencing from both forward and reverse directions.
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