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Reagents were aliquoted and each aliquot was used only once.
This bead suspension was aliquoted, 400 µl in each aliquot.
Each aliquot is for a single use.
Ideally, each aliquot is for a single use.
Subsequently, each aliquot was sub-divided into two density subgroups with half.
After incubation, each aliquot was again divided into 3 equal subgroups.
Enzyme-linked immunoassays for angiogenin, interleukin-6, and vascular endothelial growth factor were performed simultaneously on each aliquot.
Each aliquot was again divided into five parts and equilibrated for 4, 24, 48, 72, and 96 hours before freezing in an automatic freezer.
During these processes, a number of random errors might be introduced in each aliquot and in each replicate measurement.
In most experiments, each aliquot was then centrifuged and the supernatant filtered through a 0.2 μm syringe filter.
The volume of the AA solution was half of that of the AgNO3 solution for each aliquot.
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