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dN and dS were estimated from each alignments using CODEML program.
First, we established data partitions for each alignments using a greedy search in PartitionFinder v1.0.1 (Lanfear et al. 2012), with linked branch lengths, constraining the models of evolution to those available in RAxML, and using AICc for model selection (a measure of AIC corrected for small sample sizes, Hurvich and Tsai 1989).
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16 The consensus sequence for each alignment was calculated with BioEdit 7.1.9.
Sequences from individual families were aligned using MUSCLE (Edgar 2004) and each alignment was edited manually.
For each alignment result we calculate the percentage of aligned reads.
For each alignment, we examined the Ensembl genes nearby the aligning fragment in each species.
Sequences were aligned using Clustal X [ 28] and each alignment was visually inspected.
Each alignment gave us an estimate of the transformation that aligns a trajectory to its reference.
Each alignment contains core-regions that correspond to the most reliably alignable sections of the alignment.
For alignment of subsequent mask edges, a square window is added corresponding to each alignment fork.
Computation time increases significantly with the number of properties affected by each alignment.
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CEO of Professional Science Editing for Scientists @ prosciediting.com