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Unique SNPs were detected in each accession.
(a) Homozygous and heterozygous SNPs from each accession.
Prior to the diversity assessment, the geographic origin of each accession was mapped.
The correct DNA sequence was selected after comparing three plasmids from each accession.
The average trait value of each accession was used in data analyses of GWAS.
Each set of experiment had three replicates with 10 plants of each accession per replicate.
All traits were measured from 5 randomly sampled plants for each accession after harvest.
Lsmeans were computed for each accession using SAS v9.2 (SAS Institute, Cary NC, USA).
A DNA sample from each accession was then sent to Cornell University for SSR marker analysis.
For amplified fragment length polymorphism (AFLP) analysis, one individual was used from each accession.
For each accession, DNA was extracted from one seed [29].
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