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Polyethylenes (PE) with long chain branching (LCB) densities up to 0.44 carbons/10 000 candonarrow narrow molecular weight distributions were synthesized using a continuous stirred-tank reactor (CSTR) with the Dow Chemical's constrained geometry catalyst system, CGC-Ti/TPFPB/MMAO, in Isopar E solution at 500 psig and 140 190°C.
In acidic solution diluted in a mixture of water and ethanol (E solution).
Before being pipetted onto the plate, proteins in the plasma samples (20 μl) were digested by adding 2 μl 20 mg/ml Actinase E solution (Sigma-Aldrich, Sweden) in 0.5 M Tris-HCl, pH 7.4, followed by incubation for 20 h at 55 °C.
e Solution D is sterile distilled water.
After the staining procedure (D, E solution) and dehydration, the slides were coverslipped with DPX mountant for histology (VWR International).
Briefly, the liver were perfused firstly with calcium and magnesium - free solution for 10 min at 37°C, and then with Pronase E solution (2.4 mg/ml) and collagenase IV solution (0.3 0.45 mg/ml) for 10 min and 30 min respectively at 37°C, the digested liver were excised, dispersed in serum-free MEM,and filtered through gauze.
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