This is likely due to the fact that the inbreeding N e was estimated using a strict deterministic model; while the variance N e was estimated from simulations of s = 0.01, where genetic drift plays a bigger role.
The dose length product (DLP), scan acquisition time and CT dose index (CTDI) were extracted from the structured Digital Imaging and COmmunications in Medicine (DICOM) report, and dose exposure (E) was estimated using a previously published method [18].
Effective dose (E) was estimated using the DLP method [ 29].
The levels of superoxide dismutase, glutathione peroxidase, vitamin C, and vitamin E were estimated using standard procedures.
The BioRad iCycler IQ 3.1 Optical System Software was used and PCR efficiency (E) was estimated using the equation (1+E) = 10 -1/slope) [ 25].
For each primer pair, PCR efficiencies (E) were estimated using absolute fluorescence data captured during the exponential phase of amplification of each reaction with the equation (1+E) = 10 -1/slope) [ 100].
The ancestral nucleotide sequences of SFTSV lineages A, B, C and E were estimated using the ML method described above, then translated into amino acid sequences for structure-based analysis.
The expected genome length, G e, was estimated using method 4 of Chakravarti et al. (1991) [ 53], in which the total length of the linkage groups is multiplied by the factor (m + 1)/ m-1), where m is the number of markers in the linkage groups.
Variance components between genes (σ g ), between genes within array (σ ag ), between genes within dye (σ dg ), between genes within horn phenotype (σ hg ), between genes within sex (σ sg ), and within genes (σ e ) were estimated using restricted (to zero error contrasts) maximum likelihood based on the analytical gradients option of VCE6 software ftp://ftp.tzv.fal.de/pub/vce6/.de/pub/vce6/
For each trait, the broad sense heritability (h) was estimated as: (5) h 2 = σ G E 2 σ G E 2 + σ E 2 Genotypic variance (σ G E 2 ) and residual variance (σ E 2 ) were estimated using the VARCOMP procedure of SAS with the method residual maximum likelihood algorithm REML [ 31].
