The functionality of both motility systems depends on localization of proteins to the correct cell poles between reversals and their dynamic pole-to-pole relocation during reversals (Mignot et al, 2005; Leonardy et al, 2007; Bulyha et al, 2009).
FRAP analysis revealed that, while EGFP-hZW10 was highly dynamic at spindle poles in untreated cells (Figure 1D), NDGA treatment stabilized hZW10 at the spindle pole during both prometaphase and metaphase (Figure 2D).
However, this motility system also depends on polar localization of proteins between reversals and their dynamic relocalization between poles during reversals as shown for the two A-motility proteins, AglZ and RomR, which have unknown functions in A-motility.
Several experimental tests are performed to identify the dynamic model, then pole placement technique and integral control by state augmentation are employed to design the control law.
The effect of fiber type and orientation, the pole geometry, and the concentrated mass at the pole tip are evaluated by performing the dynamic analysis of FRP poles under step, triangular and sine pulses.
In the present work, a simple, but reliable, tapered beam element is developed and the dynamic behavior of the poles is accurately captured using only a few elements.
It was really interesting to see actually the dynamic change that the poles are undergoing right now, and to think about the implications that could have for the whole planet".
In these cases, the microtubules were dynamic, and the spindle poles were more dynamic when grouped in pseudo-bipolar spindles.
In this paper, an intelligent controller capable of static balancing as well as dynamic balancing of a pole mounted on a motorized robot is designed and developed.
The pole placement design methodology is applied to attain a good dynamic response by placing the poles of the sliding plane in the desired location.
