Static and dynamic modes of the decomposition were examined.
The effects of structural dynamic modes of the spindle are compensated via a proposed observer.
MC3T3 preosteoblasts were cultivated on nanograted polydimethylsiloxane substrates and a thorough quantification – in static and dynamic modes – of the morphological and structural features of focal adhesions and cytoskeleton was performed.
For example, if a protein or complex can be rotationally immobilized on a solid support, then existing CW and time-domain methods in SDSL-EPR can be implemented to reveal internal dynamic modes of the protein on the nano- to microsecond time scale, free from contributions due to overall rotary diffusion, and for determination of interspin distances at room temperature using dipolar spectroscopy.
Moreover, the single-cell data from the reporter line showed that the phenotypic outcome of arrest versus death correlated strongly with the dynamic mode of p53, as overall 86% of the cells (summed over all drug concentrations) that showed p53 pulsing went into cell-cycle arrest, and more than 96% of those with monotonic p53 induction died.
Although the dynamic mode of AFM provides better resolution than the ARS/hopping mode of SICM in some samples, only the latter can obtain contact-free images of samples with steep slopes, rendering it an important tool for observing live cells as well as unfixed or fixed soft samples with complicated shapes.
In addition, dynamic mode of measurement suffers from the limitation due to stiffness-induced shift in resonant frequency by the adsorbates apart from the mass of target molecules restricting the immobilization area which results in reduced biological sensitivity.
Our observations that p53 dynamics can switch modes in a damage dose-dependent manner, and that cell fate can be altered by changing the dynamic mode of p53, reveal a new mechanism by which the p53 pathway regulates differential DNA-damage responses.
In dynamic mode of operation, change in mass of the cantilever platform due to the addition of target molecules is measured in terms of change in its resonant frequency.
Based on the quantified p53 fluorescent trajectories, we classified the dynamic mode of nuclear p53 manually by eye.
