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The mechanism for this ambiguous, possibly dynamic localisation behaviour is unclear: as noted above, it may correlate with spontaneous activation of the GTPase cycle.
A key observation concerns the dynamic localisation of eIF2B in response to alcohols (Taylor et al., 2010).
During slow phase, Rab11-GFP exhibits dynamic localisation to, and in the vicinity of, the centrosomes apical to each nucleus (Fig. 8A; supplementary material Movies 9 and 10).
To describe the dynamic localisation of proteins in the nucleolus, we investigated the extent of nucleolar association of proteins by first collating an extensively curated literature-derived dataset.
The dynamic localisation of mitochondria was analysed in living cells after a 6-h incubation with propranolol alone, vincristine alone or their combination.
Regions of conserved sequence to either side of the relatively well-characterised core of the protein are even more mysterious, but may be important for its function in Dictyostelium, perhaps by mediating its dynamic localisation.
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(B ) Dynamic co-localisation of strain expressing Rrm4-Cherry, λN*NLS-Gfp, and cdc3B 16.
(B ) Dynamic co-localisation studies of Upa1-Gfp (left) and Rrm4-Rfp (right) as in (A ) (Video 13 ).
(I ) Dynamic co-localisation studies of Rrm4-Gfp (left) and Rab5a-Cherry (right) using dual view and msALEX microscopy (arrowheads indicate co-localising signals).
Dynamic co-localisation studies of Rrm4-Gfp (left) and (A ) Yup1-Cherry (right) or (B ) FM4-64-stained signals (right) using dual view and msALEX microscopy.
(A ) Dynamic co-localisation studies of Upa1-Gfp (left) and Rab5a-Cherry (right) using dual view and msALEX microscopy (see 'Materials and methods'; arrowheads indicate co-localising signals).
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