Sentence examples for dynamic cultivation from inspiring English sources
For dynamic cultivation, scaffolds were placed inside the bioreactor and cultivated at 37°C under continuous perfusion with addition of HEPES buffer solution (PAA laboratories, US) to the culture medium in order to maintain a stable pH.
Surface TATVHL peptide was effective in promoting the quantity of cartilaginous components in constructs after dynamic cultivation.
Recent studies indicate that phenotypic trade-offs, which are often observed after evolution under constant conditions, can be mitigated by using dynamic cultivation regimes.
A significant increase in ALP activity after 14 days of ODM was recognized in dynamic cultivation compared with that of static cultivation.
After 14 days of ODM culture, a significant increase in cell number and a higher density of cell distribution in the scaffold were observed after both static and dynamic cultivation compared to GM culture.
Cells that BMP-2 expressed were frequently observed after 14 days in dynamic culture compared with other conditions, and the expression of osteopontin was confirmed in dynamic cultivation after both 7 days and 14 days.
This is necessary to eliminate variability that is inherent in dynamic batch cultivation [ 7, 9- 11].
However, as shown in Table II, the amount of total extracellular protein for each strain at the end of the dynamic batch cultivation was basically the same, indicating that the single mutations did not cause significant problems in protein folding or secretion.
> The different P. pastoris strains carrying the respective mutated HRP gene were physiologically characterized in single dynamic batch cultivations in the controlled environment of a bioreactor.
The distinct capability of the mathematical model to predict highly dynamic fed-batch cultivation strategies was demonstrated by experimental implementation of two fed-batch cultivation strategies.
However, the cellular transcriptome of mammalian cells is very dynamic during batch cultivation where the conditions change continuously due to nutrient consumption and the accumulation of metabolites (Hernandez Bort et al. 2012; Koh et al. 2009).
