The precise intracellular visualization of Hfq and other proteins with the clonable MT tag for EM can be the first step of a series of dynamic studies based on correlative microscopy for a precise analysis of the functional localization of proteins and sRNAs in live bacteria.
Here, we show a clear causal relationship between the freeze-concentration event and particle aggregation by employing correlative imaging techniques, encompassing both real-time dynamic visualization and super-resolution imaging for frozen systems.
Thirdly, taking the first two limitations together, observations comparing absolute numbers from section to section for a process that is undergoing dynamic changes in a 10 h window are necessarily correlative.
Although not included in the study presented here, correlative imaging strategies, such as PET/CT or dynamic MRI, with the ability to link these imaging endpoints to tumor or circulating biological surrogates are feasible through the designed infrastructure.
Correlative analyses were performed using Pearson's correlation.
Remarkably, Ranchoux and co-workers applied transmission electron microscopy, and correlative light and electron microscopy, providing unequivocal ultrastructural-level evidence of ongoing dynamic EndMT in PH samples.
Thus, an integrated approach of high-resolution structure determination methods and correlative light, soft X-ray and EM allows us to look at dynamic biological processes at different resolutions and complexity.
Finally, with the development of live cell imaging methods and correlative live cell imaging EM, a unique tool is provided to integrate dynamic parameters with ultrastructural protein localization studies, which opens a whole new field of analysis.
In the statistical SW correlative study, this is manifested as generally low paired correlation coefficient values (CC < ~0.5) with individual solar wind parameters, showing comparable statistical contributions of dayside merging electric field E kl and solar wind dynamic pressure.
