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Toxicologists realise that cell-based dynamic assays can acquire important additional information about toxic responses of individual cell within a treated population.
In other words, we challenge the idea that induced thermotolerance does not occur in dynamic assays with slow heating rates.
A related but unstated presumption is that the dynamic assays of Figure 2 predict total protrusion number, and expressing Cdc42 would provide a test of that as effect of its overexpression is opposite to Rac1.
We have recently discussed why these conflicting outcomes arise, and suggest that the contribution of other stressors (e.g. higher desiccation in long thermal tolerance assays associated with slow warming rates) can potentially overshadow thermal acclimation effects in dynamic assays with varying heating rates [ 67].
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The enhanced intracellular complexity and the active exocytic dynamic assayed by our morphological evaluations similarly suggest a constitutive activated phenotype for dermal ALMS fibroblasts.
In the 96.96 BioMark dynamic assay, a Ct value of 26 corresponds to one DNA molecule in the reaction chamber.
LG-C, AF and FC performed the semen collection, processing of samples for centrifugation and cryopreservation, and helped by MAR, JG and CS performed the sDFI dynamic assay.
All strains were tested on the dynamic assay, and 17h-biofilms were stained (SYTO9, Life Technologies) and fluorescence intensity quantified by microscopy (Zeiss, ImageJ).
In contrast to microarray-based assays, qRT-PCR, in principle, provides a broader dynamic assay range and could provide a more accurate representation of CRBN mRNA transcript levels in patients.
Hence, we next examined the relation between prolonged accumulation of STL-6014 withen tumorsmons (on days five and seven post administration for small and large tumors, respectively) and tumor necrosis that was independently assessed by tumor dissection and histopathology as described above, following the whole body dynamic assay.
USA300 S. aureus strains, TCH1516 and FPR3757, were found to be closely related based on whole genome mapping (Argus™ Optical Mapping System, Opgen Inc, Gaithersburg, USA) to UAS391 (96.3-99.196.3-99.1%ity, P=0.0151), however differed markedly in biofilm formation (P=0.0001) on a dynamic assay (BioFlux 200, Fluxion Biosciences, USA).
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