Sentence examples for dynabeads for from inspiring English sources

Therefore, we stimulated CD4+CD25lo T cells with anti-CD3/CD28 Dynabeads for 48 hours and monitored the expression of FOXP3 and CD25 up to day 16 of culture (Figure 5A).

Cell lysates were incubated with 0.1% Triton X-100 for 15 min and then clarified by centrifugation at 10,000 g for 10 min. The cleared lysates were incubated at 4°C with anti-FLAG or anti-HA antibody for 1.5 h and then with Protein G-coated Dynabeads for another 1.5 h.

Briefly, PBMCs were resuspended in a small volume with biotinylated anti-CD8 monoclonal antibody (BD Biosciences), incubated for 20 min at 4°C, the excess antibody was then washed off, cells were incubated with Dynabeads for 20 min at room temperature on an orbital shaker, and separated by a magnet.

The Dynabeads/mAb complex was incubated overnight at 4°C then washed several times with PBS to remove excess mAb. 100 µl of 1% scrapie infected brain homogenate was incubated with 1×106 mAb-coated Dynabeads for at least 2 hours at room temperature.

In parallel, 2 μg of antibody was conjugated to Dynabeads for 1 hr at 4 °C with rocking.

Mononuclear cells were incubated with anti-CD3 monoclonal antibody-coated Dynabeads for 20 minutes at 4°C under constant rotation.

A certain amount (normally 0.5 1 mg) of total protein was used for IP with Abs coupled with Dynabeads (Invitrogen) for 3 hours before adding into the cell lysates and incubation overnight at 4°C with rotation.

Jurkat, JCaM2 wt LAT or CD4 T cells were incubated with anti-CD3 antibody (OKT3 -coated M450 Dynabeads (Dynal) fOKT3 -coated [30] or antibody-coated polystyrene beads [17] for M450oscopy on ice (beaDynabeads ratio 1:2) anDynalivated for the isolation time at 37°C.

Immune cells were identified and isolated using an immunomagnetic positive cell isolation procedure (Dynabeads® for Human Monocytes, Human B cells, Human CD4 cells, Invitrogen Dynal AS, Oslo, Norway).

Thereafter the cell suspensions were incubated with SE-1 antibody-tagged Dynabeads® for 20 min at 4°C in an orbital shaker.

Lysates were cleared by protein G Dynabeads (Invitrogen) for 1 hour at 4°C; 1 µl of TDP-43 antibody (anti TDP-43 C-terminus 405 414, Cosmo Bio Co). was added to each cell lysate (300 µg) and the samples were incubated for 3 hours at 4°C on a rotating wheel.