Then, deciding the images needed more color, he made vegetable dyes, mixing mustard, pimentos and coffee with Wite-Out.
So this is really really pioneering, because thus far Donald Judd had anodized and dyed his box sculptures, but to my knowledge no one else has taken the dyes, mixed their own colors, and then applied them with a brush onto the substrate. .
- We realized extra experiments for samples preparation and compared extensively direct dyes mixing with lipids and DMSO loading.
After this incubation, 10 µL of Redox Dye Mix MA (HepG2/C3A, HepG2, COLO 205, A549, PC-3) or MB (HL-60 and CCRF-CEM) was added, and cells were incubated to assess dye reduction for 5 hours (Redox Dye Mix MA) or 24 hours (Redox Dye Mix MB) and photographed.
Aliquots (100 µl) of cell suspension and 1× Biolog redox dye mix A were inoculated into each well of the plates respectively, followed by incubation at 28 °C.
Cell pellets were then re-suspended in 25-ml cold PBS, and 2 ml EB/AO (1 1, 4 mg/ml) dye mix was added.
The adherent cells were washed carefully with 1.0 mL of PBS followed by addition of 20 μL of the dye mix containing ethidium bromide (100 mg/mL) and acridine orange (100 mg/mL).
The overnight cultured bacterial colonies were inoculated into Biolog IF-0a GN/GP Base medium to reach 85%% turbidity followed by 1 200 dilution aliquoted into IF-10b medium supplemented with Dye Mix A as indicated by the manufacturer instructions.
The final concentration of dye mix was 4 µg/ml AO and 4 µg/ml EB in PBS.
After an 18-hour incubation, 10 µL of Redox Dye Mix MA was added to each well.
