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The aRNA from the same sample was labelled with Cy3 and Cy5 to test the fluorescent dyes' effect due to labelling and hybridization.
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For all dyes, effects of buffer pH on maxima and shape of both absorption and fluorescence spectra were absent or negligible (≤3 nm), emphasizing the absence of major pH-dependent effects other than PET.
#9: ADIDAS BY STELLA MCCARTNEY YOGA CLIMALITE® OMBRÉ STRETCH TANK Dip dye effect gives a fashionable edge to this cool, calm, and comfortable ombré stretch yoga tank.
Expression data from the dye-swap pairs were averaged in order to eliminate any potential gene-specific dye effect.
For each of the three pools of placental parasites, two dye swaps were performed to compensate dye effect and to assess technical reproducibility, leading to four hybridized slides.
A second degree-polynomial curve was fitted through the 16 data points while conserving the temporal order and correcting for the dye effect.
There also is a dye effect.
The dye effect usually differs for each probe.
DG ln represents the gene-specific dye effect.
Loess normalization was performed for each microarray to correct the dye effect and technical bias.
There is no dye effect using this end-labeling method [ 46].
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