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The system configuration of the industrial scale multiple supercritical CO2 apparatus and its dyeing procedure for bobbins were described for the first time.
The flow chart of dyeing procedure can be shown as-.
The silk was then dyed with indigotin using the reduction dyeing procedure, and the dyeing procedure was the same as above.
Two pieces of silk sample were dyed with berberine, palmatine, alizarin, purpurin, and indigotin consecutively, one treated with alum mordanting before each dyeing procedure and the other treated with iron sulfate mordanting before each dyeing procedure.
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Numerous developments in dyeing context are observed in recent decades such as evaluation of effective mordants, printing techniques and dyeing procedures [34, 35, 36].
Follows is an abbreviated presentation of the pre-test, scouring, mordanting, and dyeing procedures; detailed procedures are presented by Doty (2015).
Endothelial cells were stained for alkaline phosphatase (AP) using an azo-dye procedure.
Total protein concentration in each pooled CSF sample was determined by the Bio-Rad protein assay (Bio-Rad Laboratories, HerCAles, CA, USA), based on the Bradford dye procedure.
Table 2 summarizes the amount of protein applied, number of spots resolved, differences observed, and number of proteins identified for each dye procedure used.
The dishes were then subjected to the multiple washing procedure described above and the number of cells remaining in the dishes was measured by the neutral red dye procedure as described previously.
A flip labeling (dye-swap or reverse labeling with Cy3 and Cy5 dyes) procedure was followed to nullify the dye bias associated with unequal incorporation of the two Cy dyes into cDNA (Rosenzweig et al., 2004; Altman, 2005; Martin-Magniette et al., 2005).
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