Several approaches have been described in vivo, including the use of non-specific dye tracking (e.g. for detection of non-specific inflammatory sites), the use of fluorophores tagged-antibody-based targets for specific cell molecules and/or metabolic pathways and the development of fluorescence reporter proteins (e.g. GFP) for the detection of gene expression.
Specificity of dye tracking may be enhanced by utilising an antibody, or its fragment, directed at a specific molecular target.
PCR amplification and detection are performed in a single tube, where probes with four different reporter dyes track the different targets in the multiplex reaction: (i) HPV 16, and 18 individually, (ii) 12 HR-HPV types (i.e., HPV 31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, and −68) as a group, and (iii) beta-globin.
Using the two dye tracks as guides, the gel fragment between them can be excised from the post-run gel prior to any staining for DNA visualization.
However, cell dilution dye assays tracking proliferation of Tregs and Teff cells during suppression indicated increased cell cycling from the entire population of Tregs in SFM making outgrowth of a small subset during the culture an unlikely explanation for the increased proliferation observed.
To explore the nature of degradation of the PA-RGDS hydrogel in vivo, we labeled PA-RGDS with CM-DiI, a red fluorescent dye, for tracking gels and intramyocardially injected it into the hearts of C57B6 mice where myocardial infarction was induced by ligation of the left anterior descending coronary artery.
Our results promote a new way of designing versatile, efficient and convenient quadruplex-reporting dyes for tracking these higher-order nucleic acid structures in living human cells.
Finding out whether the heart is actually short of blood and at high risk for an attack requires tests other than a CT scan — most likely, a nuclear stress test, which uses radioactive dye to track blood flow through the coronary arteries.
The release into the media from a specially prepared tablet containing Rhodamine 6G dye was tracked in time and the areal distribution method developed by Alberini et al. (2014a) was implemented to characterise the mixing performance.
In a separate group, the cells were stained with calcein dye to track the viable cells.
In future studies this could determined by intravenous injection of an endothelial cell marker (eg., tomato lectin) or a fluorescent dye to track perfusion.
